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Using Pipettes - Importance of Pipette Calibration
The pipette is a common instrument used in science labs and heath care centers. This instrument creates a vacuum to draw liquid and so it is used for measuring or transferring materials. The accuracy of this instrument measurements is very crucial and therefore, pipette calibration on regular basis is necessary. Get your instruments calibrated regularly if you wish that your instruments give accurate measurements.
Can You Calibrate Pipettes On Your Own?
You can get your pipette instruments calibrated on your own if you consider certain things into your mind. There are certain things which you will need while doing calibration of your instruments like pipette, thermometer, Erlenmeyer flask, pipette filler, beaker, weight balance, and pen & paper. Get hold of all the necessary items which are required for the purpose of calibration. Once you get them, you can start doing the process easily.
Getting Started:
1. Cleaning the Equipments
First of all, clean and dry your pipette and beaker which you are going to use. By cleaning equipments, any residue from your past use will be removed which can skew your measurements. It is very crucial step you have to do before you go further.
2. Working with Erlenmeyer Flask
You have to put distilled water in your Erlenmeyer flask and leave it for 15 minutes. Waiting for 15 minutes, you can measure the temperature of the water to see the difference.
3. Using Beaker and Weight Balance
You have to first determine the mass of the beaker and it should be around tenth of a milligram. Here, you will need weight balance to do perform this task.
4. Using Pipette Filler
Using pipette filler, you have to fill your pipette with the waster taking from the flask. Then, you have to put the water into the beaker you have taken. After that, weigh the beaker to see the difference in weight from your previous measurements. To arrive at a fine conclusion, you have to repeat this process three or four times.
As you go further doing all necessary steps, as mentioned so far, you have to determine the density of water at the same temperature you measured previously. After that, you have to find the mean volume of water discharged by your pipette by using the formula volume = density/mass. You may compare your calculations or measurements with other results for pipette calibration so that you can have the precision for your pipette instrument.
These are some of the tips and steps you may consider while doing pipette calibration on your own. If you wish to learn more about calibration services consisting of instrument calibration and temperature calibration, you may visit rscalibration.com.
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